Protein purification service is a critical step in many experiments. It separates proteins from other molecules in a sample, allowing scientists to study the protein’s structure, function and interactions with other biological molecules. The process is especially important for structural, enzyme and protein-protein interaction studies, which require a pure sample to avoid interference from contaminants.
Once a protein is isolated, it can then be used in a variety of downstream applications. These include research on a protein’s structure and function, as well as developing new disease treatments using the protein’s specific amino acid sequence or binding affinity. Purified proteins are also used to validate a protein’s expression and characterization, and to verify its purity before use in experiments.
Efficient Protein Purification Services: What to Expect
In addition to protein purification, the R&D Systems MagneHis(tm) system can be used for protein conjugation, allowing you to turn your recombinant protein into a reagent or diagnostic tool for your specific experiment. Conjugating the polyhistidine tag on a protein with a fluorophore, biotin or other chemical label enables it to be easily tracked and analyzed by a range of techniques.
Protein purification utilizes differences in a protein’s size, physicochemical properties, or charge to separate it from other proteins. Separation steps can be performed by centrifugation, SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) or by chromatography. The latter technique uses a chromatography resin with oppositely charged ions to separate the protein sample into fractions that bind and elute with increasing strength of charge.
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